Binding of leu5 -enkephalin and met5 -enkephalin to a particulate fraction from rat cerebrum
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چکیده
منابع مشابه
Binding of Leu5-enkephalin and Met5-enkephalin to a particulate fraction from rat cerebrum.
Leucine-enkephalin and methionine-enkephalin are two pentapeptides with morphine like properties, naturally present in mammalian brain. They were first characterized by Hughes et al. [ 1 ] as H-TyrGlyGlyPheLeu-OH and H-TyrGlyGlyPheMet-OH respectively and found to behave as potent, although short lived opiates in a variety of pharmacological tests in vivo [2]. They do interact with the so-called...
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Regulation of cellular content of the endogenous opioid peptides Met5-enkephalin and Leu5-enkephalin was investigated in neuroblastoma X glioma hybrid cells NG108-15 grown in both serum-supplemented and serum-free defined media. Untreated cells and cells induced to differentiate were stained using anti-Met5-enkephalin and anti-Leu5-enkephalin with the peroxidase-antiperoxidase immunocytochemica...
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The influence of chronic activation of gamma-aminobutyric acid (GABA) system on Met5-enkephalin (ME) biosynthesis was investigated in male rats. Activation of GABA system was achieved by raising the brain GABA concentration with aminooxyacetic acid (AOAA) or gabaculine which inhibits GABA-transaminase the enzyme responsible for the catabolism of GABA. After a regimen of repeated administration ...
متن کامل[Leu5]enkephalin-encoding sequences are targets for a specific DNA-binding factor.
A DNA-binding factor with high affinity and specificity for the [Leu5]enkephalin-encoding sequences in the prodynorphin and proenkephalin genes has been characterized. The factor has the highest affinity for the [Leu5]-enkephalin-encoding sequence in the dynorphin B-encoding region of the prodynorphin gene, has relatively high affinity for other [Leu5]enkephalin-encoding sequences in the prodyn...
متن کاملIn vitro release of [5-methionine]enkephalin and [5-leucine]-enkephalin from the rat globus pallidus.
Endogenous [5-methionine]enkephalin (Metenkephalin) and [5-leucine]enkephalin (Leu-enkephalin) are released from perfused slices of rat globus pallidus by increased K(+) in a Ca(2+)-dependent manner. Tissue perfused for 40 min contained only 26% of the Met-enkephalin and 44% of the Leu-enkephalin found in the freshly dissected tissue. After perfusion, the mean (+/-SEM) ratio (wt/wt) of Met-enke...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80236-6